A new pair of primers for molecular sexing of the Pallas squirrel, Callosciurus erythraeus, and variation in fetal sex ratio

Publication Type:Journal Article
Year of Publication:2006
Authors:Kusahara, M, Kamimura, Y, Tamura, N, Hayashi, F
Journal:Mammal Study
Volume:31
Pagination:87-92
Date Published:2006
ISBN Number:1343-4152
Keywords:Callosciurus erythraeus
Abstract:

ABSTRACT The Pallas squirrel, Callosciurus erythraeus, is an introduced species to Japan where a male-biased sex ratio has been reported at the weaning stage in the field. For further examination of their earlier (fetal) sex ratio, a new PCR primer set was designed to amplify a portion of the Sry (sex-determining region Y) gene. This successfully produced sex-specific PCR profiles for known-sex adult samples. Using this technique we could determine the sex of fetal samples dissected out of mothers shot as pests on Izu-Oshima Island, Tokyo, Japan. The fetal sex ratio was significantly male-biased in 2000, but not in other years. The sex ratio tending towards male-bias in this squirrel is discussed from a local resource competition theory.

URL:http://dx.doi.org/10.3106/1348-6160(2006)31[87:ANPOPF]2.0.CO;2
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