Carnivora: the amino-acid sequence of the adult Sumatran tiger (Panthera tigris sumatrae) hemoglobins

Publication Type:Journal Article
Year of Publication:1989
Authors:Jahan, M, Ahmed, A, Braunitzer, G, Goltenboth, R
Journal:Biological Chemistry Hoppe-Seyler
Volume:370
Date Published:1989
Keywords:CARNIVORA, Panthera tigris
Abstract:

The complete amino-acid sequences of the hemoglobins from the adult Sumatran tiger (Panthera tigris sumatrae) have been determined on automatic liquid- and gas-phase sequenators. The globin chains were isolated by reverse phase HPLC on a column of Nucleosil-C4. N-Acetylserine was detected by FAB-mass spectroscopy as N-terminal aminoacid residue of the beta I chain. Comparing the sequences of the globin chains of the tiger with that of human Hb-A, 23 substitutions were recognized in the alpha, 29 in beta I and 28 in the beta II chain.

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